Anti-inflammatory properties of Holothuria atra extract on lipopolysaccharide induced inflammation of White blood cells

Awad, Safaa Nageb; Abd El Hafez, Mohammed Said; Abd Elwahab, Miral Gamal; Mady, Rehab; Batiha, Gaber El-Saber; Shaheen, Hazem M; Ghareeb, Doaa Ahmed

doi:10.21608/djvs.2022.157375.1090

Anti-inflammatory properties of Holothuria atra extract on lipopolysaccharide induced inflammation of White blood cells

Document Type : Original Article

Authors

¹ Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt

² National Institute of Oceanography and Fisheries (NIOF), Cairo, Egypt

³ Center of Excellence for Drug Preclinical Studies (CE DPS), Pharmaceutical and Fermentation Industries Development Centre (PFIDC), City of Scientific Research and Technological Applications (SRTA-City), New Borg El Arab, Egypt

⁴ Bio screening and Preclinical Trial Lab, Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt

10.21608/djvs.2022.157375.1090

Abstract

Holothuria atra is a marine invertebrate found in the tropical indo-pacific region. It contains potent bioactive compounds, which include lectin, steroidal saponins, and triterpene glycoside. The anti-inflammatory and antioxidant characteristics of Holothuria atra extract have been investigated in-vitro in this study. Ethyl acetate extract of H. atra was prepared by maceration methods, and then the phytochemical compositions were measured colorimetric and by using gas chromatography-mass spectrometry (GC-MS) Analysis. Hemolytic activity and anti-inflammatory properties of the extract were measured through cell culture protocol. The obtained results showed that the extract has a high phenol content (121.92±0.14 mg/g), and GC-MS analysis revealed that it contains, Oleic acid, 9-octadecenoic acid, methyl ester, and Octadecanoic acid.
Moreover, antioxidant properties were determined by assessing 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, ferric reducing power, hydroxyl radical scavenging, and American Board of Thoracic Surgery (ABTS) scavenging activity. Furthermore, it is concluded that H. atra inhibited the stimulated Lipopolysaccharide (LPS) - Inducible nitric oxide synthase (iNOS) gene expression and protein expression by 6.17±0.01-fold change and Cyclooxygenase-2 (COX-2) gene expression by 4.70±0.05-fold change. The crude extract of H. atra could be considered a good anti-inflammatory agent.

Keywords