Validation of Lyophilized-Attenuated Single and Combined Canine Hepatitis, Canine Distemper, and Canine Parvovirus Vaccines

Abdel-Samad, Asmaa Gamal; Bkear, Nabil Mohamed; Khadr, Adel Mohamed; Khodier, Mohamed Hassan; Edries, Samir Mohamed

doi:10.5455/djvs.2021.80072.1039

Validation of Lyophilized-Attenuated Single and Combined Canine Hepatitis, Canine Distemper, and Canine Parvovirus Vaccines

Document Type : Original Article

Authors

¹ Agriculture Research Center (ARC); Veterinary Serum and Vaccine Research Institute (VSVRI), Abasia, Cairo, Egypt

² Faculty of Veterinary Medicine, Damanhour University, Damanhour, Egypt

³ Faculty of Veterinary Medicine, Alexandria University, Egypt

10.5455/djvs.2021.80072.1039

Abstract

Through this study, we attempted to prepare and evaluate lyophilized attenuated single and trivalent vaccines of Canine Distemper (CD), Canine Adeno-1 (CA-1), and Canine Parvo (Cp type 2a) viruses. All such preparations were found to be free from foreign contaminants, safe in mice and puppies, and potent, providing vaccinated puppies with good levels of specific antibody titers against the three viruses as estimated by serum neutralization test (SNT) and indirect immune sorbent assay (ELISA). Such antibodies were found to be stable up to 12 months post-vaccination, supporting their use to protect puppies against the three virus infections with the preferable use of the trivalent vaccine, which saves effort and avoids stress factors of vaccinated puppies.

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