Document Type : Original Article
Authors
1
Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour ,Egypt
2
Head of the inspection department on the campus and butcher shops
3
Prof. of Biotechnology ,Department of Medical Biotechnology, Institute of Genetic Engineering ,City of Scientific Research and Biotechnological Applications
4
Department of pharmacology, faculty of veterinary medicine, Damanhour University, Damanhour, Egypt
5
associate professor Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt
6
Senior of Department of Pharmacology, Faculty of Veterinary Medicine, Damanhour University, El-Gomhoreia street, Damanhour, El-Behera Governorate, Egypt.
Abstract
Silymarin, a flavonoid derived from plants, has emerged as a potential neuroprotective agent against Multiple neurological conditions, as Alzheimer’s disease, cerebral ischemia, and Parkinson's disease. Fish-scale collagen, known for its high biodegradability, excellent biocompatibility, and low antigenicity, was utilized in this study to create collagen nanoparticles for neuroprotection drug delivery. The investigation aimed to extract Mullet scale-derived collagen and produce collagen nanoparticles using ethanol as an insoluble solvent. Silymarin Was incorporated into the nanoparticles via the nanoparticle precipitation method. MTT assay was employed to compare the cytotoxicity of Collagen nanoparticles loaded with silymarin with that of free silymarin. Remarkably, no statistically significant change in cytotoxicity was observed in cells that have been served by silymarin rates lower than 100 µg/ml. The study successfully demonstrated the effective loading of silymarin into collagen nanoparticles, these findings were confirmed by transmission electron microscopy (TEM) and dynamic light scattering (DLS) ,(TEM), technique on a Zeta sizer Nano ZS, scanning electron microscope, also FTIR is used for measuring the radiation absorption of infrared regions at various wavelengths. Notably, the cytotoxic effects of silymarin followed a consistent pattern at all concentrations: silymarin loading in collagen nanoparticles < silymarin nanoparticles < free silymarin. Also, we demonstrate the therapeutic impact of silymarin nanoparticle and collagen nanoparticle on antioxidant biomarkers in brain tissues of AlCl3 induced Alzheimer's that that confirmed by the histological analysis of the brain that show substantial neuroprotective of silymarin plus collagen nanoparticles, this suggests that loading silymarin into collagen nanoparticles is both efficient and safe.
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